Competencies

Application of Genetic Engineering Methods

Primer design and cloning using PCR

Cloning in E. coli and K. phaffii
Fermentation of E. coli, S. sp., K. phaffii and various ascomycetes

Small scale (1 mL)

Laboratory scale up to 20 L

Recombinant protein synthesis
Application of Protein-Chemical Methods

Fluorescence spectroscopy

Circular dichroism spectroscopy

Microscale thermophoresis

Purification of Proteins using FPLC

Immobilized metal affinity chromatography (IMAC)

Anion exchange chromatography (AEC)

Size exclusion chromatography (SEC)
Application of Bioanalytical Methods

Tricine-/SDS-PAGE and native PAGE

Western blot and dot blot
Univariate and multivariate data analysis

Python

R Studio

Flow cytometry

Measurement of the metabolic activity of yeasts

Determination of yeast cell age

Quantification of mixed fermentations

Cell cycle analysis
Particle and Protein Size Measurement

DLS (Dynamic Light Scattering)

SLS (Static light scattering)

FT-IR (Fourier-Transform Infrared Spectroscopy)
Synthesis and Functionalization of Magnetic Nanoparticles and Surfaces

Coating of quartz glass surfaces with EDTA

Silanization of particles and surfaces with subsequent immobilization
Microscopy

Scanning Electron Microscopy (SEM)

Confocal Laser Scanning Microscopy (CLSM)

Zur Verfügung gestellt von L. Wittmann/STT/TU München.