About Me - Marco Eigenfeld

MARCO EIGENFELD

Dr.-Ing. in Bioprocess Engineering/Biotechnology

With a solid academic background in bioengineering and bioprocess engineering, I possess extensive expertise in the fermentation and purification of proteins. In my previous role as a research assistant at the Technical University of Munich, I was actively involved in cloning for recombinant protein synthesis, as well as the subsequent fermentation and purification processes. During my studies, I developed the ability to design innovative process concepts that prioritize both sustainability and economic efficiency—skills I am currently applying in my postdoctoral research at the Medical University of Graz in Austria.

In addition to my technical skills, I bring a high level of dedication, curiosity, and motivation, which enable me to quickly and effectively adapt to new responsibilities. I am also experienced in managing projects and collaborating with interdisciplinary teams.

Contact

Awards

MY KNOWLEDGE

Protein chemistry 85%

Microbiology 75%

Nanobiotechnology 80%

R Studio 75%

Python/PyCharm 70%

Molecularbiology 85%

MY EXPERIENCE

07/2024 - Present

Deputy Head of "Nanomaterials for Drug Delivery"

Medical University of Graz, Chair of Medicinal Chemistry
Main areas of focus:
· Project management
· Project aquisition
· Mentorship of students work

07/2024 - Present

Lab manager "NanoLab Graz"

Medical University of Graz, Chair of Medicinal Chemistry
Main areas of focus:
· Development of protein tags for oriented immobilization of proteins
· Characterization of these proteins with regard to secondary structure and affinity
· Cultivation of bacteriophages
· Protein synthesis in E. coli and P. pastoris
· Functionalization of nanoparticles

07/2024 - Present

Postdoctoral Researcher

Medical University of Graz, Chair of Medicinal Chemistry
Main Research Interests:
· Cloning and expression of binding proteins
· Characterization of these proteins for secondary structure and affinity
· Fractionation of heterogeneous yeast cell populations based on cell age
· Supervision of PhD students and theses
· Project management
· Acquisition of third-party funding

01/2024 - 06/2024

Scholarship (Ernst Mach Worldwide - OeAD)

Medical University of Graz, Chair of Medicinal Chemistry

08/2022 - 12/2023

Deputy Group Leader

Technical University of Munich, Chair of Brewing and Beverage Technology, RG Beverage and Cereal Biotechnology
Responsibilities:
· Project management
· Acquisition of third-party funding

01/2022 - 12/2023

Head of Microbiology Laboratory

Technical University of Munich
Microbiology Laboratory and contract analytical services:
· Microscopic analyses and detection of contaminations and foreign yeasts in liquid samples
· Identification through sequencing
· Determination of the physiological state of yeasts
· Application-oriented basic research on brewer's and baker's yeast, as well as pharmaceutically relevant yeasts

11/2022 - 12/2023

Lecturer at the Doemens Academy

Lecturer in the Chemical Technician Course with a focus on process engineering

09/2019 - 12/2023

Lecturer, Doemens Academy

Lecturer in the Laboratory Technician Course with a focus on antibodies and immunoassays

10/2016 - 12/2023

Research Assistant

Technical University of Munich, Chair of Brewing and Beverage Technology
Main Focus of Work:
· Application of genetic engineering methods for recombinant protein synthesis
· Implementation of fermentations on a laboratory scale up to 20 liters
· Flow cytometry (CytoFlex, Beckman Coulter)
· Multivariate data analysis using R and Python
· Purification of proteins using FPLC (ÄKTA pure)
· Application of various bioanalytical methods, including Tricine-/SDS-PAGE, native PAGE, Western blot, and dot blot
· Application of various protein-chemical methods, including fluorescence spectroscopy, circular dichroism spectroscopy, and microscale thermophoresis (NanoTemper)
· Particle and protein size measurement using DLS and SLS
· Synthesis and functionalization of magnetic nanoparticles
· Visualization using CLSM and SEM

04/2015 - 10/2015

Research Assistant

RPTU Kaiserslautern, Chair of Bioprocess Engineering
Main Focus of Work:
· Biocatalysis of recombinantly produced enzymes
· Immobilization of enzymes on glass surfaces and superparamagnetic nanoparticles

MY EDUCATIONAL PATH

2016 - 2023

Technical University of Munich

Doctorate in Engineering Sciences
Doctoral Thesis:
"Effect of the singular cell age on Saccharomyces pastorianus ssp. carlsbergensis proliferation"

2014 - 2016

RPTU Kaiserslautern

Master of Science in Bioprocess Engineering
Master's Thesis:
"Measurement method for enzyme cascade reactions in microreactors and determination of enzymatic/non-enzymatic activation energy"

2010 - 2014

TH Bingen am Rhein

Bachelor of Science in Biotechnik
Bachelor's Thesis:
"Comparative study of optimization models for increasing production of antifungal agents from Penicillium sp. IBWF047-10 in liquid media"

MY REFERENCES

Dr. Anita Emmerstorfer-Augustin

Ass. Prof. Sebastian Schwaminger

Prof. Sandro Keller