Competencies

  • Application of genetic engineering methods
    Primer design and cloning using PCR
    Cloning in E. coli and K. phaffii
  • Fermentation of E. coli, S. sp., K. phaffii and various ascomycetes
    Small scale (1 mL)
    Laboratory scale up to 20 L
    Recombinant protein synthesis
  • Application of protein-chemical methods
    Fluorescence spectroscopy
    Circular dichroism spectroscopy
    Microscale thermophoresis
  • Purification of proteins using FPLC
    Immobilized metal affinity chromatography (IMAC)
    Anion exchange chromatography (AEC)
    Size exclusion chromatography (SEC)
  • Application of bioanalytical methods
    Tricine-/SDS-PAGE and native PAGE
    Western blot and dot blot
  • Univariate and multivariate data analysis
    Python
    R Studio
  • Flow cytometry
    Measurement of the metabolic activity of yeasts
    Determination of yeast cell age
    Quantification of mixed fermentations
    Cell cycle analysis
  • Particle and protein size measurement
    DLS (Dynamic Light Scattering)
    SLS (Static light scattering)
    FT-IR (Fourier-Transform Infrared Spectroscopy)
  • Synthesis and functionalization of magnetic nanoparticles and surfaces
    Coating of quartz glass surfaces with EDTA
    Silanization of particles and surfaces with subsequent immobilization
  • Microscopy
    Scanning Electron Microscopy (SEM)
    Confocal Laser Scanning Microscopy (CLSM)

Zur Verfügung gestellt von L. Wittmann/STT/TU München.