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Application of genetic engineering methods
Primer design and cloning using PCRCloning in E. coli and K. phaffii -
Fermentation of E. coli, S. sp., K. phaffii and various ascomycetes
Small scale (1 mL)Laboratory scale up to 20 LRecombinant protein synthesis -
Application of protein-chemical methods
Fluorescence spectroscopyCircular dichroism spectroscopyMicroscale thermophoresis
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Purification of proteins using FPLC
Immobilized metal affinity chromatography (IMAC)Anion exchange chromatography (AEC)Size exclusion chromatography (SEC) -
Application of bioanalytical methods
Tricine-/SDS-PAGE and native PAGEWestern blot and dot blot -
Univariate and multivariate data analysis
PythonR Studio
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Flow cytometry
Measurement of the metabolic activity of yeastsDetermination of yeast cell ageQuantification of mixed fermentationsCell cycle analysis -
Particle and protein size measurement
DLS (Dynamic Light Scattering)SLS (Static light scattering)FT-IR (Fourier-Transform Infrared Spectroscopy) -
Synthesis and functionalization of magnetic nanoparticles and surfaces
Coating of quartz glass surfaces with EDTASilanization of particles and surfaces with subsequent immobilization -
Microscopy
Scanning Electron Microscopy (SEM)Confocal Laser Scanning Microscopy (CLSM)